Science & Health

Microdosing at Night: Does Psilocybin Affect Sleep?

Psilocybin mushrooms enhance creativity and focus, so microdosing at night is not recommended.

Min read
Cori Sue Morris
November 30, 2022

Microdosing at Night: Will it keep you awake?

So, you’re considering a microdosing regime. Your afternoons are a bit low energy, so you’re considering microdosing at night. But, you’re wondering how it will affect your already tenuous relationship with sleep? We feel you.

With cell phone addiction, global anxiety and depression on the rise, a war in Ukraine, and not to mention the current “vibe recession,” it’s a wonder any of us can get any sleep at all.

You’re probably sitting here wondering if microdosing psilocybin at night will make you more alert, or sleepy, like marijuana often can.

Well, we have great news for you. Psilocybin, the chemical compound in magical mushrooms, increases dopamine and serotonin receptors in the brain, and, when properly microdosed, users report increased focus, enhanced creativity, and improved mood.

Psychedelics, like psilocybin, increase neuroplasticity, or the brain’s ability to  change and to create new neurons.

Microdosing at Night: the Science of Psilocybin Mushrooms

So while psilocybin is not considered a stimulant like amphetamines, studies show it can make you more alert and increase neural activity in the brain. So— to best take advantage of psilocybin’s benefits —it’s best taken first thing in the morning as you prepare to meet the challenges and opportunities of the day.

That said, does taking psilocybin in the morning to improve alertness and productivity impact your sleep at night, hours later? According to recent studies, the answer is no. Researchers at the University of Oxford recently studied the effects of psilocybin in mice. They found that mice that were administered psilocybin experienced delayed REM sleep for approximately 3 hours, but did not experience long-term changes in sleep quantity.

Psilocybin’s ability to increase dopamine and serotonin - without impacting our sleep patterns - is one of the reasons we’re seeing increased curiosity from the scientific community, the general public, and even President Biden, about the benefits of psilocybin as a medical treatment for depression and other mental health conditions.

Curious about microdosing?

Learn to microdose with our free guide.

Thank you! Check your email to begin your journey
Oops! Something went wrong while submitting the form.

Remind me: What do Dopamine & Serotonin do?

Quick biology 101 refresh: Dopamine is our body’s naturally occurring neurotransmitter that is also known as the feel good hormone, as dopamine levels directly correlate with happiness and, conversely, depression. Serotonin is another chemical in our nervous system that affects our emotions of pleasure, joy and reward, and also affects digestion and metabolism.

Recent Research Studies on Psilocybin & Sleep

Researchers at the National Institute of Mental Health in Prague, Czechoslovakia, are studying psilocybin’s antidepressant properties and the effects of psilocybin on sleep. In a recent study published in 2020, researchers conducted a double blind study on humans to evaluate the effect of psilocybin on sleep architecture on the night after psilocybin administration.

Researchers gathered subjective (participant’s feedback) and objective (via computing) assessments of sleep: sleep latency (the time it takes you to fall asleep), total sleep time, sleep efficiency, and the number of sleep cycles. By both subjective and objective measures, none of these metrics were significantly different in placebo and psilocybin conditions.

All in all, studies to date suggest that microdosing psilocybin is a great way to start your day, as it can lead to improved brain function, creativity, and mood. It can increase alertness, so it’s best to microdose in the morning. And, to date, studies have not found negative, long-term implications on sleep.

In sum: we do not recommend microdosing at night.

Microdosing & Reduced Caffeine Intake  

Caffeine is the original drug of choice for many people—the fuel of late-stage capitalism.  Most of us know that drinking coffee in the afternoon can seriously impact sleep. In fact, studies show the the half-life of coffee—or the the time it takes to metabolize the coffee—is 5.7 hours for the average, healthy adult.

Many people turn to microdosing as an alternative solution to coffee in order to increase productivity and creativity, without the jitters. And, early findings suggest that people who microdose mushrooms drink less caffeine throughout the day.  

Less coffee is a great thing for those looking to improve their sleep quality.

Importantly, be sure to consult a healthcare provider or professional, to follow a proper microdosing protocol, and, as always, to start low and go slow, when implementing a microdosing practice.

Keep reading

Discover the latest psychedelic news and guidance from psychedelic experts.

Back to Journal
About the Author

Cori Sue Morris

Cori Sue is the founder & CEO of Retreat. She’s a recovering journalist and serial entrepreneur, having started, scaled and sold a media company, Bitches Who Brunch, and a marketing agency. She loves mushrooms, microdosing, empowered women, and helping others.

REad more
Mushroom Curious?

Optimize your wellbeing with guided microdosing

Discover our comprehensive, science-backed microdosing programs and resources, designed to help you make change guided by experienced psychedelic & medical experts.



Take our research-backed, expert-led microdosing course and learn to enhance your wellbeing and improve mental health.

Start Now


Meet 1:1 with a microdosing coach and get the support you need to unlock change, driving creativity, focus, and joy.

Start Now


A private, invite-only space for information, community, entrepreneurship, and collaboration for the mushroom-curious.

Join Us

Curious about microdosing?

Learn to microdose with our free guide.

Microdosing 101

Benefits and side effects of Microdosing

How to microdose

Microdosing protocol

Flow State

Psychedelics & the Future of Health

Thank you! Check your email to begin your journey
Oops! Something went wrong while submitting the form.

Join us @retreatmicrodose